IDENTIFICATION OF THE COAT PROTEIN GENE OF BEAN GOLDEN MOSAIC GEMINIVIRUS

被引：3

作者：

MURAYAMA, A ^{[1
]}

YAMAYA, Y ^{[1
]}

IKEGAMI, M ^{[1
]}

机构：

[1] TOKYO UNIV AGR, NODAI RES INST, SERAGAYA KU, TOKYO 156, JAPAN

来源：

INTERVIROLOGY | 1991年 / 32卷 / 04期

关键词：

BEAN GOLDEN MOSAIC GEMINIVIRUS; WHITEFLY-TRANSMITTED PLANT VIRUS; COAT PROTEIN GENE; PKK223-3 EXPRESSION VECTOR; IMMUNOBLOT ASSAY;

D O I：

10.1159/000150208

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An open-reading frame designated 1R1, on DNA component 1 of bean golden mosaic geminivirus (BGMV), has been identified as the coat protein gene. A DraI restriction fragment of BGMV DNA 1 that includes 1R1 was inserted into the SmaI pKK223-3 expression vector. The 32 kD protein expressed in Escherichia coli cells reacted with antibodies to the BGMV capsid polypeptide and behaved identically to purified capsid protein in western blots.

引用

页码：258 / 261

页数：4

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