OPTIMUM STAINING OF PCNA IN PARAFFIN SECTIONS IS DEPENDENT ON FIXATION, DRYING, AND INTENSIFICATION

被引：33

作者：

GOLICK, ML ^{[1
]}

RICE, M ^{[1
]}

机构：

[1] COULTER IMMUNOL,HIALEAH,FL 33010

来源：

JOURNAL OF HISTOTECHNOLOGY | 1992年 / 15卷 / 01期

关键词：

IMMUNOPEROXIDASE; IMIDAZOLE; PARAFFIN SECTIONS; PCNA;

D O I：

10.1179/his.1992.15.1.39

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Variable expression of proliferating cell nuclear antigen (PCNA) with immunoperoxidase staining in formalin fixed paraffin embedded tissue is dependent on fixation length, temperature of section drying, and intensity of substrate staining. This study showed that after 24 hr fixation, the tissue is "overfixed." Optimum staining of PCNA was achieved by drying paraffin sections at low temperatures and by increasing DAB staining with imidazole plus a metallic stain such as osmium tetroxide.

引用

页码：39 / 41

页数：3