CLONING AND EXPRESSION OF THE BOVINE SODIUM-DEPENDENT AND CHLORIDE-DEPENDENT NORADRENALINE TRANSPORTER

被引:79
作者
LINGEN, B [1 ]
BRUSS, M [1 ]
BONISCH, H [1 ]
机构
[1] UNIV BONN,INST PHARMAKOL & TOXIKOL,D-53113 BONN,GERMANY
关键词
NORADRENALINE TRANSPORTER; PLASMA MEMBRANE; ADRENAL MEDULLA; PROTEIN KINASE C;
D O I
10.1016/0014-5793(94)80508-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cDNA encoding a functional bovine, tricyclic antidepressant-sensitive noradrenaline transporter has been identified by screening a lambda gt11 cDNA library of the bovine adrenal medulla using the cDNA of the human noradrenaline transporter [1991, Nature 350, 350-354]. The sequence predicts a protein of 615 amino acids (M(r) 68 900). The bovine transporter shares 93% amino acid identity with the human sequence, but displays two more consensus sites for phosphorylation by protein kinase C. Transient expression of the transporter in COS-7 cells resulted in a sodium- and chloride-dependent uptake of noradrenaline with a pharmacology typical for a neuronal noradrenaline transporter.
引用
收藏
页码:235 / 238
页数:4
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