CLONING AND EXPRESSION OF THE BOVINE SODIUM-DEPENDENT AND CHLORIDE-DEPENDENT NORADRENALINE TRANSPORTER

被引：79

作者：

LINGEN, B ^{[1
]}

BRUSS, M ^{[1
]}

BONISCH, H ^{[1
]}

机构：

[1] UNIV BONN,INST PHARMAKOL & TOXIKOL,D-53113 BONN,GERMANY

来源：

FEBS LETTERS | 1994年 / 342卷 / 03期

关键词：

NORADRENALINE TRANSPORTER; PLASMA MEMBRANE; ADRENAL MEDULLA; PROTEIN KINASE C;

D O I：

10.1016/0014-5793(94)80508-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding a functional bovine, tricyclic antidepressant-sensitive noradrenaline transporter has been identified by screening a lambda gt11 cDNA library of the bovine adrenal medulla using the cDNA of the human noradrenaline transporter [1991, Nature 350, 350-354]. The sequence predicts a protein of 615 amino acids (M(r) 68 900). The bovine transporter shares 93% amino acid identity with the human sequence, but displays two more consensus sites for phosphorylation by protein kinase C. Transient expression of the transporter in COS-7 cells resulted in a sodium- and chloride-dependent uptake of noradrenaline with a pharmacology typical for a neuronal noradrenaline transporter.

引用

页码：235 / 238

页数：4

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