IDENTIFICATION OF A SATELLITE DOUBLE-STRANDED-RNA IN THE PARASITIC PROTOZOAN TRICHOMONAS-VAGINALIS INFECTED WITH T-VAGINALIS VIRUS T1

Co-infection by a 0.5-kb small double-stranded (ds) RNA together with Trichomonas vaginalis virus (TVV) genomic 4.6-kb dsRNA is commonly observed in a number of T. vaginalis isolates. By molecular cloning and primer extension experiments, the 497-bp cDNA sequence of a 0.5-kb dsRNA co-infecting with TVV-T1 in T. vaginalis T1 isolate was elucidated. Consistent with the replication cycle of a typical dsRNA virus, a plus-strand viral RNA beginning at +1 of the 0.5-kb dsRNA was identified in infected T. vaginalis T1 cells by primer extension and Northern hybridization studies. The 0.5-kb dsRNA was separately encased in TVV capsids from the viral genomic dsRNA, as shown by protein analysis and electron microscopic examination of Viral particles purified by multiple rounds of CsCI gradient centrifugation. The riboprobes transcribed from a cloned cDNA of the 0.5-kb dsRNA exhibited strong hybridization to a small dsRNA in a T. vaginalis T9 isolate, which harbors a NV-TS distantly related to TVV-T1, but the same probes showed very little hybridization to the viral genomic dsRNA of both NV-TI and NV-TS. Very little sequence homology between the 0.5-kb dsRNA and the 4.6-kb dsRNA in TVV-T1 was found by computer-assisted analysis, suggesting that the small dsRNA in T. vaginalis T1 is not derived from the genome of TVV-T1 or other distantly related T. vaginalis viruses. These results suggest that the small dsRNAs in T. vaginalis are satellite RNAs of T. vaginalis virus. (C) 1995 Academic Press, Inc.