SPECTROPHOTOMETRIC DETERMINATION OF MYCELIAL BIOMASS

被引：29

作者：

BANERJEE, UC ^{[1
]}

CHISTI, Y ^{[1
]}

MOOYOUNG, M ^{[1
]}

机构：

[1] UNIV WATERLOO,DEPT CHEM ENGN,WATERLOO N2L 3G1,ONTARIO,CANADA

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 04期

关键词：

D O I：

10.1007/BF00150905

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The optical density (450 nm) of samples of homogenized fungal biomass correlated linearly with the dry weight of the biomass in the samples. As shown for broths of the filamentous microfungus Neurospora sitophila, the sensitivity of the technique depended on the extent of fragmentation of fungal hyphae during homogenization: increased fragmentation increased sensitivity. The method applied during all phases of growth, was as accurate as the conventional dry weight technique and permitted rapid and simple measurement of biomass concentration.

引用

页码：313 / 316

页数：4

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