RFLP MARKERS TO IDENTIFY THE ALLELES ON THE MLA LOCUS CONFERRING POWDERY MILDEW RESISTANCE IN BARLEY

被引：50

作者：

SCHULLER, C ^{[1
]}

BACKES, G ^{[1
]}

FISCHBECK, G ^{[1
]}

JAHOOR, A ^{[1
]}

机构：

[1] TECH UNIV MUNICH,LEHRSTUHL PFLANZENBAU & PFLANZENZUCHTUNG,WEIHENSTEPHAN 12,W-8050 FREISING,GERMANY

来源：

THEORETICAL AND APPLIED GENETICS | 1992年 / 84卷 / 3-4期

关键词：

RFLP MARKER; BARLEY; POWDERY MILDEW; MLA LOCUS;

D O I：

10.1007/BF00229491

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

To identify the mildew resistance locus Mla in barley with molecular markers, closely linked genomic RFLP clones were selected with the help of near-isogenic lines having the 'Pallas' and 'Siri' background. Out of 22 polymorphic clones 3 were located around the Mla locus on chromosome 5 with a distance of 5.1 +/- 2.9 cM (MWG 1H068), 4.2 +/- 1.7 cM (MWG 1H060) and 0.7 +/- 0.7 cM (MWG 1H036), respectively. The polymorphic clone MWG 1H036 displayed the same RFLP pattern in both 'Pallas' and 'Siri' near-isogenic lines and in different varieties digested with six restriction enzymes possessing the same mildew resistance gene. The alleles of the Mla locus were grouped in 11 classes according to their specific RFLP patterns; 3 of these groups contain the majority of Mla alleles already used in barley breeding programs in Europe.

引用

页码：330 / 338

页数：9

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