METALLOPROTEINASES IN THE MINERALIZED COMPARTMENTS OF PORCINE DENTIN AS DETECTED BY SUBSTRATE-GEL ELECTROPHORESIS

被引：25

作者：

FUKAE, M ^{[1
]}

KANEKO, I ^{[1
]}

TANABE, T ^{[1
]}

SHIMIZU, M ^{[1
]}

机构：

[1] TSURUMI UNIV,SCH DENT MED,DEPT BIOCHEM,YOKOHAMA,KANAGAWA,JAPAN

来源：

ARCHIVES OF ORAL BIOLOGY | 1991年 / 36卷 / 08期

关键词：

PORCINE DENTIN; METALLOPROTEINASE; SUBSTRATE-GEL ELECTROPHORESIS; DEGRADATION OF NONCOLLAGENOUS PROTEINS;

D O I：

10.1016/0003-9969(91)90106-5

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Several gelatinolytic activities were detected in the 4 M guanidine HCl-soluble fraction extracted from demineralized porcine dentine matrix. These matrix proteinases were active in the range of pH 6-9, and the activities were enhanced by calcium ions. The enzymes also degraded some of the non-collagenous proteins coexisting in the extract. The results indicate that the changes in the composition of non-collagenous proteins during the development of dentine are related to proteolytic enzyme activity.

引用

页码：567 / 573

页数：7

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