PREFERENTIAL BINDING OF H1E HISTONE TO GC-RICH DNA

被引：27

作者：

WELLMAN, SE ^{[1
]}

SITTMAN, DB ^{[1
]}

CHAIRES, JB ^{[1
]}

机构：

[1] UNIV MISSISSIPPI,MED CTR,DEPT BIOCHEM,JACKSON,MS 39216

来源：

BIOCHEMISTRY | 1994年 / 33卷 / 01期

关键词：

D O I：

10.1021/bi00167a049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the binding of a pure H1 histone, the mouse variant H1e, to a 214 bp fragment of DNA from pBR322. Binding was monitored by observing the effects of the protein on melting of the DNA and by a gel-mobility-shift assay. We found, using this highly purified system, that H1e protein binds preferentially and cooperatively to the GC-rich region of the DNA. A chemically synthesized peptide containing 25 residues, corresponding to a region of the carboxyl-terminal domain of H1e, shows the same sequence preference but does not exhibit cooperativity.

引用

页码：384 / 388

页数：5

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