TRANSLATION OF THE MESSENGER-RNA OF THE MAIZE TRANSCRIPTIONAL ACTIVATOR OPAQUE-2 IS INHIBITED BY UPSTREAM OPEN READING FRAMES PRESENT IN THE LEADER SEQUENCE

被引:99
作者
LOHMER, S
MADDALONI, M
MOTTO, M
SALAMINI, F
THOMPSON, RD
机构
[1] MAX PLANCK INST ZUCHTUNGSFORSCH,CARL VON LINNE WEG 10,W-5000 COLOGNE 30,GERMANY
[2] IST SPERIMENTALE CEREALICOLTURA,I-24100 BERGAMO,ITALY
关键词
D O I
10.1105/tpc.5.1.65
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The protein encoded by the Opaque-2 (02) gene is a transcription factor, translated from an mRNA that possesses an unusually long 5' leader sequence containing three upstream open reading frames (uORFs). The efficiency of translation of O2 mRNA has been tested in vivo by a transient assay in which the level of activation of the b32 promoter, a natural target of 02 protein, is measured. We show that uORF-less O2 alleles possess a higher transactivation value than the wild-type allele and that the reduction in transactivation due to the uORFs is a cis-dominant effect. The data presented indicate that both uORF1 and uORF2 are involved in the reducing effect and suggest that both are likely to be translated.
引用
收藏
页码:65 / 73
页数:9
相关论文
共 45 条
  • [1] THE B-32 PROTEIN FROM MAIZE ENDOSPERM, AN ALBUMIN REGULATED BY THE O2 LOCUS - NUCLEIC-ACID (CDNA) AND AMINO-ACID SEQUENCES
    DIFONZO, N
    HARTINGS, H
    BREMBILLA, M
    MOTTO, M
    SOAVE, C
    NAVARRO, E
    PALAU, J
    RHODE, W
    SALAMINI, F
    [J]. MOLECULAR & GENERAL GENETICS, 1988, 212 (03): : 481 - 487
  • [2] ELGIN SCR, 1975, METHOD ENZYMOL, V40, P158
  • [3] AMBER CODON SUPPRESSION - THE INVIVO AND INVITRO ANALYSIS OF 2 C-HORDEIN GENES FROM BARLEY
    ENTWISTLE, J
    KNUDSEN, S
    MULLER, M
    CAMERONMILLS, V
    [J]. PLANT MOLECULAR BIOLOGY, 1991, 17 (06) : 1217 - 1231
  • [4] PRIMARY STRUCTURE OF A C-HORDEIN GENE FROM BARLEY
    ENTWISTLE, J
    [J]. CARLSBERG RESEARCH COMMUNICATIONS, 1988, 53 (04) : 247 - 258
  • [5] THE O2 GENE WHICH REGULATES ZEIN DEPOSITION IN MAIZE ENDOSPERM ENCODES A PROTEIN WITH STRUCTURAL HOMOLOGIES TO TRANSCRIPTIONAL ACTIVATORS
    HARTINGS, H
    MADDALONI, M
    LAZZARONI, N
    DIFONZO, N
    MOTTO, M
    SALAMINI, F
    THOMPSON, R
    [J]. EMBO JOURNAL, 1989, 8 (10) : 2795 - 2801
  • [6] STRUCTURE AND FUNCTION OF SUPPRESSOR TRANSFER-RNAS IN HIGHER EUKARYOTES
    HATFIELD, DL
    SMITH, DWE
    LEE, BJ
    WORLAND, PJ
    OROSZLAN, S
    [J]. CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1990, 25 (02) : 71 - 96
  • [7] INVOLVEMENT OF AN INITIATION-FACTOR AND PROTEIN-PHOSPHORYLATION IN TRANSLATIONAL CONTROL OF GCN4 MESSENGER-RNA
    HINNEBUSCH, AG
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (04) : 148 - 152
  • [9] Jefferson RA, 1987, PLANT MOL BIOL REP, V5, P387, DOI DOI 10.1007/BF02667740
  • [10] AFFECTING GENE-EXPRESSION BY ALTERING THE LENGTH AND SEQUENCE OF THE 5' LEADER
    JOHANSEN, H
    SCHUMPERLI, D
    ROSENBERG, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (24): : 7698 - 7702