DETERMINATION OF HUMAN URINARY HYALURONIC-ACID, CHONDROITIN SULFATE AND DERMATAN SULFATE AS THEIR UNSATURATED DISACCHARIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：58

作者：

TOYODA, H ^{[1
]}

MOTOKI, K ^{[1
]}

TANIKAWA, M ^{[1
]}

SHINOMIYA, K ^{[1
]}

AKIYAMA, H ^{[1
]}

IMANARI, T ^{[1
]}

机构：

[1] CHIBA UNIV,FAC PHARMACEUT SCI,CHIBA 260,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 565卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80378-P

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of hyaluronic acid (HA), chondroitin sulphate (CS) and dermatan sulphate (DS) was developed. HA, CS and DS were converted to the corresponding unsaturated disaccharides by digestion with chondroitinase ABC and/or chondroitinase AC-II and determined by high-performance liquid chromatography with fluorimetric detection using 2-cyanoacetamide as a post-column derivatization reagent. The calibration graphs for the unsaturated disaccharides were linear over the range 2 ng-2-mu-g for each unsaturated disaccharide. This method was applied to the analysis of normal human urine.

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页码：141 / 148

页数：8

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