CALIBRATION FOR INSTRUMENTAL SPREADING IN SIZE EXCLUSION CHROMATOGRAPHY BY A NOVEL RECYCLE TECHNIQUE

被引：13

作者：

ALBA, D ^{[1
]}

MEIRA, GR ^{[1
]}

机构：

[1] INTEC,CC 91,RA-3000 SANTA FE,ARGENTINA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1986年 / 9卷 / 06期

关键词：

CHEMICAL ANALYSIS - Equipment;

D O I：

10.1080/01483918608075496

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

So far, the best available techniques for the instrumental spreading calibration in size exclusion chromatography (SEC) prvide the first moments of the spreading function, and require the measurement of several recycles. This work presents a method that enables the calculation of the spreading, making no 'a priori' assumptions with regards to its shape, from the information contained in the original and first recycle chromatograms of a narrowly distributed standard only. Then, the corrected chromatogram may be obtained. The technique is tested on experimental data.

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收藏

页码：1141 / 1161

页数：21

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