NALOXONE-INDUCED ANOREXIA INCREASES NEUROPEPTIDE-Y CONCENTRATIONS IN THE DORSOMEDIAL HYPOTHALAMUS - EVIDENCE FOR NEUROPEPTIDE-Y OPIOID INTERACTIONS IN THE CONTROL OF FOOD-INTAKE

被引：15

作者：

LAMBERT, PD ^{[1
]}

WILDING, JPH ^{[1
]}

ALDOKHAYEL, AAM ^{[1
]}

GILBEY, SG ^{[1
]}

GHATEI, MA ^{[1
]}

BLOOM, SR ^{[1
]}

机构：

[1] HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,DIV ENDOCRINOL & METAB,LONDON W12 0NN,ENGLAND

来源：

PEPTIDES | 1994年 / 15卷 / 04期

关键词：

NEUROPEPTIDE Y; NALOXONE; HYPOTHALAMUS; FOOD INTAKE;

D O I：

10.1016/0196-9781(94)90091-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We measured neuropeptide Y (NPY) concentration in microdissected hypothalamic nuclei, by radioimmunoassay, and NPY mRNA in the hypothalamus in rats treated systemically with the nonspecific opioid antagonist, naloxone, to produce mild anorexia. Twenty rats were treated with daily SC injections of naloxone (7.5 mg/kg); 20 were treated with vehicle alone. Naloxone produced a 7% reduction in food intake (P < 0.01) and a reduction in weight gain (p < 0.002). Neuropeptide Y concentrations were increased specifically in the dorsomedial nucleus of the hypothalamus (DMN) in rats treated with naloxone (6.8 +/- 0.7 fmol/mu g protein vs. 3.1 +/- 1.0 fmol/mu g protein, p < 0.05, n = 10 per group). Total hypothalamic NPY mRNA was unchanged. Neuropeptide Y-opioid interactions may be important in the control of food intake.

引用

页码：657 / 660

页数：4

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