DISTRIBUTION OF X-RAY-INDUCED G2 CHROMATID DAMAGE AMONG CHINESE-HAMSTER CHROMOSOMES - INFLUENCE OF CHROMATIN CONFORMATION

被引：55

作者：

SLIJEPCEVIC, P

NATARAJAN, AT

机构：

[1] LEIDEN STATE UNIV,MGC DEPT RADIAT GENET & CEHM MUTAGENESIS,2333 AL LEIDEN,NETHERLANDS

[2] JA COHEN INTER UNIV INST RADIOTHERAPY & RADIAT PR,LEIDEN,NETHERLANDS

来源：

MUTATION RESEARCH | 1994年 / 323卷 / 03期

关键词：

X-RAY INDUCED CHROMATID DAMAGE; CHROMATIN CONFORMATION; CHINESE HAMSTER FIBROBLASTS;

D O I：

10.1016/0165-7992(94)90084-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Exponentially growing primary embryonic Chinese hamster cells, in which the pattern of distribution of heterochromatin is well characterized, were X-irradiated and fixed at 1, 2, 3, and 4 h following irradiation. In one set of cells repair of damage was completely blocked by ara A. The frequencies of chromatid breaks and exchanges were evaluated for individual chromosomes. An analysis of observed and estimated expected frequencies of chromosomal aberrations indicated that in general, (a) the initial damage was higher in euchromatic regions than the heterochromatic regions and (b) the repair of DNA lesions (as evaluated by the frequencies of chromatid gaps and breaks) was more efficient in euchromatic regions than heterochromatic regions.

引用

页码：113 / 119

页数：7

共 16 条

[1]

BEDFORD JS, 1991, 9TH P ICRR C, V2, P212

[2] DNA-REPAIR IN AN ACTIVE GENE - REMOVAL OF PYRIMIDINE DIMERS FROM THE DHFR GENE OF CHO CELLS IS MUCH MORE EFFICIENT THAN IN THE GENOME OVERALL [J].