THE EFFECTS OF ULTRAVIOLET-C ON IN-VITRO NUCLEOSOME ASSEMBLY AND STABILITY

被引：13

作者：

MATSUMOTO, H

TAKAKUSU, A

OHNISHI, T

机构：

[1] NARA MED UNIV,DEPT ANAT,KASHIHARA,NARA 634,JAPAN

[2] NARA MED UNIV,DEPT BIOL,KASHIHARA,NARA 634,JAPAN

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1994年 / 60卷 / 02期

关键词：

D O I：

10.1111/j.1751-1097.1994.tb05079.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of ultraviolet C (UVC) irradiation on nucleosome assembly and its stability were investigated quantitatively using an in vitro nucleosome assembly system comprising a plasmid DNA of pBR322 and core histones isolated from rat ascites hepatoma cells. Nucleosomal formation was estimated by analyzing the resulting DNA supercoils. When UVC-irradiated (3000 J/m(2)) DNA was used as a substrate for the nucleosome assembly system, the nucleosomal formation efficiency was reduced by half compared with nonirradiated DNA. On the other hand, when the reconstituted nucleosomes (minichromosomes) on the nonirradiated DNA were irradiated with UVC (3000 J/m(2)), about half each were disrupted and retained. These results indicate that it is difficult for UV-damaged DNA regions to supercoil around the histone octamers to form nucleosomes and that the histone octamers in the UV- damaged nucleosomes tend to be dissociated from DNA.

引用

页码：134 / 138

页数：5

共 28 条

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