RAPID EFFECTS OF EGF ON CYTOSKELETAL STRUCTURES AND ADHESIVE PROPERTIES OF HIGHLY METASTATIC RAT MAMMARY ADENOCARCINOMA CELLS

被引：48

作者：

LICHTNER, RB ^{[1
]}

WIEDEMUTH, M ^{[1
]}

NOESKEJUNGBLUT, C ^{[1
]}

SCHIRRMACHER, V ^{[1
]}

机构：

[1] SCHERING AG,RES LABS,W-1000 BERLIN 65,GERMANY

来源：

CLINICAL & EXPERIMENTAL METASTASIS | 1993年 / 11卷 / 01期

关键词：

CYTOSKELETON; EGF METASTASIS;

D O I：

10.1007/BF00880072

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In the highly metastatic rat mammary adenocarcinoma cell clone MTLn3, EGF induced increased adhesion to fibronectin while in the human epidermoid carcinoma cell line A431 EGF induced diminished adhesive properties. Flattening of cells with extensive formation of filopodia was observed in MTLn3 cells within 5 min of EGF addition, while in A431 cells EGF induced rounding up and only occasional formation of filopodia. Immunofluorescent analysis revealed extension of microtubules (MT) into the filopodia and Western blot analysis demonstrated an EGF-induced 2- to 3-fold increase in the amount of assembled tubulin in MTLn3 but not in A431 cells. In MTLn3, but only marginally in A431 cells, EGF treatment resulted in phosphorylation of a 280 kD cytoskeleton-associated protein, which was rapid and dose-dependent. These results suggest differential signal transduction pathways of cytoskeleton-associated EGFRs in highly metastatic MTLn3 as compared with A431 cells.

引用

页码：113 / 125

页数：13

共 63 条

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