Differences in cycling performance have been observed in cyclists with similar VO(2)max values yet different lactate thresholds. The purpose of the current study was to compare a simulated 16.1-km cycling time trial, VO(2)max and related factors in cyclists who significantly varied in ventilatory threshold. From an original group of 18 category III or IV cyclists, two groups of 6 cyclists were formed based on ventilatory threshold values as high (77 +/- 4 % of VO(2)max - Group H) or low (68 +/- 2.8 % - Group L). VO(2)max and a 16.1-km time trial were completed on a Velodyne trainer. No significant difference (p greater than or equal to 0.05) was noted between groups in VO(2)max (Group H 4.00 +/- 0.28 l. min(-1), Group L 4.15 +/- 0.67 l.min(-1)), however significant differences (p less than or equal to 0.05) were found in ventilatory threshold and time trial scores. Group H completed the time trial in 16.29 +/- 2.08 min while Group L averaged 20.93 +/- 3.03 min. Group H completed the time trial 28% more quickly by working at a significantly higher percentage of VO(2)max, a higher power output and a faster pedal rate than Group L. From a battery of physiologic and body composition parameters, the ventilatory threshold expressed as VO2 (l.min(-1)) was the best predictor (r = -0.76) of time trial performance in the 12 cyclists. The findings of this study indicate that the ventilatory threshold was superior to VO(2)max in discerning performance differences in a 16.1-km cycling time trial, and was the best predictor of the simulated time trial performance.