ESTABLISHMENT OF A TYPING ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR FOOT-AND-MOUTH-DISEASE ANTIGEN, USING REAGENTS AGAINST VIRUSES ENDEMIC IN THAILAND

被引：5

作者：

BLACKSELL, SD

LUNT, RA

CHAMNANPOOD, C

LINCHONGSUBONGKOCH, W

NAKARUNGKUL, N

GLEESON, LJ

MEGKAMOL, C

机构：

[1] Australian Animal Health Laboratory, Commonwealth Scientific and Industrial Research Organisation, Geelong, Victoria

来源：

REVUE SCIENTIFIQUE ET TECHNIQUE DE L OFFICE INTERNATIONAL DES EPIZOOTIES | 1994年 / 13卷 / 03期

关键词：

ELISA; FOOT AND MOUTH DISEASE; THAILAND; VIRUS TYPING;

D O I：

10.20506/rst.13.3.792

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Antisera were produced at a central laboratory in Thailand against the endemic serotypes (O, A and Asia 1) of foot and mouth disease (FMD) virus. At a regional veterinary laboratory, these antisera were used in an indirect sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and serotyping of FMD virus (FMDV) antigen. ELISA readings of <0.10 optical density (OD) units were considered negative. This was verified using fifty tissue samples which were known to be negative for FMDV. The highest mean sample value for three different dilutions was 0.02 OD units. Of a total of 93 samples submitted for antigen typing, 80 (86%) tested positive by ELISA and 13 (14%) were negative. No FMDV was detected in ELISA-negative samples following attempted tissue-culture virus isolation.

引用

页码：701 / 709

页数：9