FLUORESCENCE LABELING WITH EUROPIUM CHELATE OF BETA-DIKETONES AND APPLICATION IN TIME-RESOLVED FLUOROIMMUNOASSAYS (TR-FIA)

被引：27

作者：

CI, YX

YANG, XD

CHANG, WB

机构：

[1] Department of Chemistry, Peking University, Beijing

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 179卷 / 02期

关键词：

BETA-DIKETONE; MULTIPLE LABELING; TIME-RESOLVED FLUOROIMMUNOASSAY;

D O I：

10.1016/0022-1759(94)00289-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Five beta-diketone derivatives were studied for multiple labelling of proteins. The labelled proteins were characterized by absorption and fluorescence measurements. It was found that proteins labelled with chlorosulfonylthenoyltrifluoroacetone (CTTA) were able to form highly fluorescent complexes with Eu3+ which exhibited prolonged fluorescence whereas the Eu3+ complex of hydrolyzed CTTA exhibited almost no fluorescence, and so unreacted ligand gave no background signal in immunoassays even if it was not removed from the labelled reagent. The effect of labelling on the biological activity of albumin and polyclonal antibody was studied and it was also shown that the new probe could be used in time-resolved fluorescence immunoassays.

引用

页码：233 / 241

页数：9

共 24 条

[1]

CHAN MA, 1987, CLIN CHEM, V33, P2000

[2]

CHRISTOPOULOS TK, 1990, CLIN CHEM, V36, P1497

[3] FLUORESCENCE ENHANCEMENT OF THE EUROPIUM(III) - THENOYLTRIFLUOROACETONE - TRIOCTYLPHOSPHINE OXIDE TERNARY COMPLEX BY GADOLINIUM(III) AND ITS APPLICATION TO THE DETERMINATION OF EUROPIUM(III) [J].