CONDITIONED APOMORPHINE-INDUCED TURNING IN 6-OHDA-LESIONED RATS

被引:16
作者
HUDSON, JL
FONG, CS
BOYSON, SJ
HOFFER, BJ
机构
[1] UNIV COLORADO,HLTH SCI CTR,DEPT PHARMACOL,DENVER,CO 80262
[2] UNIV COLORADO,HLTH SCI CTR,NEUROSCI TRAINING PROGRAM,DENVER,CO 80262
[3] TAICHUNG VET GEN HOSP,DEPT NEUROL,TAICHUNG,TAIWAN
关键词
ROTATION; PARKINSONS DISEASE; LEARNING; CLASSICAL CONDITIONING; DOPAMINE; SUBSTANTIA NIGRA; STRIATUM;
D O I
10.1016/0091-3057(94)90469-3
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
Apomorphine-induced turning has been used to evaluate the extent of unilateral nigrostriatal denervation after 6-hydroxydopamine (6-OHDA) lesions and subsequent functional striatal reinnervation by catecholaminergic grafts. It has been noted that the pregraft rotational pattern is usually double peaked and that fetal ventral mesencephalic grafts or dopaminergic drugs will alter the second peak but leave the first relatively unchanged. We hypothesized that the first peak may be the result of factors extrinsic to the nigrostriatal dopamine system, specifically a conditioned turning response, and would, therefore, be unperturbed by the above treatments which increase dopaminergic (DA) inputs. This was investigated by injecting 6-OHDA, unilaterally, into the nigrostriatal pathway of several groups of young Fisher 344 rats. One experimental group was repeatedly tested with 0.05 mg/kg apomorphine and the rotations quantified. A second group received similar injections of apomorphine but were prevented from rotating. Vehicle control animals were also studied for both of the above experimental groups. Subsequent to the above treatment, all animals were tested unrestrained repeatedly on apomorphine. Our results support the conditioned response hypothesis in that the first peak is not present with the initial unrestrained apomorphine behavioral trial but is present upon the second and subsequent unrestrained trials. Moreover, the restrained but apomorphine-injected rats, as well as the control animals, manifest no first peak upon their first freely moving apomorphine test; the second and subsequent unrestrained apomorphine trials, in these groups, do manifest a first peak. We conclude that the first peak represents respondently (Pavlovian) conditioned rotations and is, therefore, an indirect secondary behavioral result of unilateral nigrostriatal dopaminergic denervation and repeated apomorphine administration in an environment allowing unimpeded movements. These rotations are unlikely to be directly related to the cellular changes induced by dopaminergic manipulations in this system and, therefore, their presence in studies of striatal denervation and reinnervation using apomorphine-induced turning behavior should be interpreted accordingly.
引用
收藏
页码:147 / 154
页数:8
相关论文
共 20 条
  • [1] RECONSTRUCTION OF THE NIGROSTRIATAL DOPAMINE PATHWAY BY INTRA-CEREBRAL NIGRAL TRANSPLANTS
    BJORKLUND, A
    STENEVI, U
    [J]. BRAIN RESEARCH, 1979, 177 (03) : 555 - 560
  • [2] CONDITIONED-RESPONSE TO APOMORPHINE IN NIGRO-STRIATAL SYSTEM-LESIONED RATS - THE ORIGIN OF UNDRUGGED ROTATIONAL RESPONSE
    BURUNAT, E
    CASTRO, R
    DIAZPALAREA, MD
    RODRIGUEZ, M
    [J]. LIFE SCIENCES, 1987, 41 (15) : 1861 - 1866
  • [3] APPLICATION OF THE UNILATERAL 6-HYDROXYDOPAMINE RAT MODEL OF ROTATIONAL BEHAVIOR TO THE STUDY OF CONDITIONED DRUG EFFECTS
    CAREY, RJ
    [J]. JOURNAL OF NEUROSCIENCE METHODS, 1988, 22 (03) : 253 - 261
  • [4] CONDITIONED ROTATIONAL BEHAVIOR IN RATS WITH UNILATERAL 6-HYDROXYDOPAMINE LESIONS OF THE SUBSTANTIA-NIGRA
    CAREY, RJ
    [J]. BRAIN RESEARCH, 1986, 365 (02) : 379 - 382
  • [5] PARTIAL LESION OF THE SUBSTANTIA-NIGRA - RELATION BETWEEN EXTENT OF LESION AND ROTATIONAL BEHAVIOR
    CARMAN, LS
    GAGE, FH
    SHULTS, CW
    [J]. BRAIN RESEARCH, 1991, 553 (02) : 275 - 283
  • [6] SURVIVAL OF IMPLANTED FETAL DOPAMINE CELLS AND NEUROLOGIC IMPROVEMENT 12 TO 46 MONTHS AFTER TRANSPLANTATION FOR PARKINSONS-DISEASE
    FREED, CR
    BREEZE, RE
    ROSENBERG, NL
    SCHNECK, SA
    KRIEK, E
    QI, JX
    LONE, T
    ZHANG, YB
    SNYDER, JA
    WELLS, TH
    RAMIG, LO
    THOMPSON, L
    MAZZIOTTA, JC
    HUANG, SC
    GRAFTON, ST
    BROOKS, D
    SAWLE, G
    SCHROTER, G
    ANSARI, AA
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1992, 327 (22) : 1549 - 1555
  • [7] 18-MONTH COURSE OF 2 PATIENTS WITH GRAFTS OF FETAL DOPAMINE NEURONS FOR SEVERE PARKINSONS-DISEASE
    HOFFER, BJ
    LEENDERS, KL
    YOUNG, D
    GERHARDT, G
    ZERBE, GO
    BYGDEMAN, M
    SEIGER, A
    OLSON, L
    STROMBERG, I
    FREEDMAN, R
    [J]. EXPERIMENTAL NEUROLOGY, 1992, 118 (03) : 243 - 252
  • [8] CORRELATION OF APOMORPHINE-INDUCED AND AMPHETAMINE-INDUCED TURNING WITH NIGROSTRIATAL DOPAMINE CONTENT IN UNILATERAL 6-HYDROXYDOPAMINE LESIONED RATS
    HUDSON, JL
    VANHORNE, CG
    STROMBERG, I
    BROCK, S
    CLAYTON, J
    MASSERANO, J
    HOFFER, BJ
    GERHARDT, GA
    [J]. BRAIN RESEARCH, 1993, 626 (1-2) : 167 - 174
  • [9] A 16-CHANNEL AUTOMATED ROTOMETER SYSTEM FOR RELIABLE MEASUREMENT OF TURNING BEHAVIOR IN 6-HYDROXYDOPAMINE LESIONED AND TRANSPLANTED RATS
    HUDSON, JL
    LEVIN, DR
    HOFFER, BJ
    [J]. CELL TRANSPLANTATION, 1993, 2 (06) : 507 - 514
  • [10] HUDSON JL, 1991, SOC NEUR ABSTR, V17