INDUCTION OF A CYCLIC AMP-RESPONSIVE GENE IN LIVING CELLS REQUIRES THE NUCLEAR FACTOR CREB

被引：51

作者：

MEINKOTH, JL

MONTMINY, MR

FINK, JS

FERAMISCO, JR

机构：

[1] UNIV CALIF SAN DIEGO, CTR CANC, LA JOLLA, CA 92093 USA

[2] SALK INST BIOL STUDIES, CLAYTON FDN LABS PEPTIDE BIOL, LA JOLLA, CA 92037 USA

[3] HARVARD UNIV, MASSACHUSETTS GEN HOSP, SCH MED, MOLEC NEUROBIOL LAB, BOSTON, MA 02114 USA

[4] HARVARD UNIV, SCH MED, DEPT NEUROL, BOSTON, MA 02114 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1991年 / 11卷 / 03期

关键词：

D O I：

10.1128/MCB.11.3.1759

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We constructed cell lines containing various enhancer elements cloned upstream from a marker gene. By microinjection of specific antibodies directed against transcriptional activator proteins into these cell lines, we have developed a functional assay for factors which regulate the activity of target promoters. Here we show that microinjection of a highly specific antibody to the cyclic AMP enhancer element-binding (CREB) protein diminishes gene expression in response to cyclic AMP in living fibroblasts.

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页码：1759 / 1764

页数：6

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