PREPARATIVE LIQUID-CHROMATOGRAPHY .1. INFLUENCE OF COLUMN EFFICIENCY ON OPTIMUM INJECTION CONDITIONS UNDER ISOCRATIC ELUTION

Using a chromatographic simulation algorithm based on the Craig machine, CRAIGSIM, to simulate the chromatogram of a binary mixture, the optimum injection conditions corresponding to the maximum recovered amount of the solute of interest were found to depend on the column efficiency. Generally, the optimum injection concentration is an increasing function of the plate number. Consequences of this behaviour on the scale-up procedure in preparative liquid chromatography are discussed.