MONOCLONAL-ANTIBODIES FOR DETECTION OF NORWALK VIRUS-ANTIGEN IN STOOLS

被引：32

作者：

HERRMANN, JE

BLACKLOW, NR

MATSUI, SM

LEWIS, TL

ESTES, MK

BALL, JM

BRINKER, JP

机构：

[1] UNIV MASSACHUSETTS, SCH MED, DIV INFECT DIS & IMMUNOL, WORCESTER, MA 01655 USA

[2] STANFORD UNIV, DEPT MED GASTROENTEROL, STANFORD, CA 94304 USA

[3] BAYLOR COLL MED, DIV MOLEC VIROL, HOUSTON, TX 77030 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 09期

关键词：

D O I：

10.1128/JCM.33.9.2511-2513.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Monoclonal antibodies against the prototype 8FIIa strain of Norwalk virus were prepared and applied to an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. The monoclonal antibodies immunoprecipitated a 58-kDa protein which had been produced by in vitro transcription-translation of Norwalk virus cloned cDNA, and they reacted by EIA with recombinant Norwalk virus capsid protein at a sensitivity level of 1 ng/ml. The EIA detected virus in all tested samples from 15 different Norwalk virus-infected volunteers. No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses.

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页码：2511 / 2513

页数：3

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