DETERMINATION OF CATECHOLAMINES IN SINGLE ADRENAL-MEDULLARY CELLS BY CAPILLARY ELECTROPHORESIS AND LASER-INDUCED NATIVE FLUORESCENCE

被引：134

作者：

CHANG, HT

YEUNG, ES

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,US DOE,AMES LAB,AMES,IA 50011

[2] IOWA STATE UNIV SCI & TECHNOL,DEPT CHEM,AMES,IA 50011

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 06期

关键词：

D O I：

10.1021/ac00102a010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The present study demonstrates that native fluorescence detection combined with capillary electrophoresis separation at low pH provides high sensitivity (down to nanomolar), high resolution, high speed, and low interference for the analysis of catecholamines. Further, this method has been employed successfully for the measurement of the amounts of epinephrine and norepinephrine in individual bovine adrenal medullary cells. Application of this method to the study of neurochemistry is promising.

引用

页码：1079 / 1083

页数：5

共 46 条

[1] ON-COLUMN DIGESTION OF PROTEIN FOR PEPTIDE-MAPPING BY CAPILLARY ZONE ELECTROPHORESIS WITH LASER-INDUCED NATIVE FLUORESCENCE DETECTION
CHANG, HT
YEUNG, ES
[J]. ANALYTICAL CHEMISTRY, 1993, 65 (20) : 2947 - 2951
[2] DIRECT OBSERVATION OF EPINEPHRINE AND NOREPINEPHRINE COSECRETION FROM INDIVIDUAL ADRENAL-MEDULLARY CHROMAFFIN CELLS
CIOLKOWSKI, EL
COOPER, BR
JANKOWSKI, JA
JORGENSON, JW
WIGHTMAN, RM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (08) : 2815 - 2821
[3] QUANTITATIVE-DETERMINATION OF CATECHOLAMINES IN INDIVIDUAL BOVINE ADRENOMEDULLARY CELLS BY REVERSED-PHASE MICROCOLUMN LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION
COOPER, BR
JANKOWSKI, JA
LESZCZYSZYN, DJ
WIGHTMAN, RM
JORGENSON, JW
[J]. ANALYTICAL CHEMISTRY, 1992, 64 (06) : 691 - 694
[4] GREENBERG A, 1982, CELL TISSUE RES, V266, P655
[5] CATECHOLAMINE SECRETION BY ISOLATED ADRENAL-CELLS
HOCHMAN, J
PERLMAN, RL
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 421 (01) : 168 - 175
[6] DETERMINATION OF INTRACELLULAR SPECIES AT THE LEVEL OF A SINGLE ERYTHROCYTE VIA CAPILLARY ELECTROPHORESIS WITH DIRECT AND INDIRECT FLUORESCENCE DETECTION
HOGAN, BL
YEUNG, ES
[J]. ANALYTICAL CHEMISTRY, 1992, 64 (22) : 2841 - 2845
[7] ULTRASENSITIVE ENZYME-IMMUNOASSAY
ISHIKAWA, E
HASHIDA, S
KOHNO, T
HIROTA, K
[J]. CLINICA CHIMICA ACTA, 1990, 194 (01) : 51 - 72
[8] JANKOWSKI JA, 1992, J BIOL CHEM, V267, P18329
[9] MEASUREMENT OF PLASMA SEROTONIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION AS AN INDEX OF THE INVIVO ACTIVITY OF FLUVOXAMINE
KEATING, J
DRATCU, L
LADER, M
SHERWOOD, RA
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 615 (02): : 237 - 242
[10] MICROCOLUMN SEPARATIONS AND THE ANALYSIS OF SINGLE CELLS
KENNEDY, RT
OATES, MD
COOPER, BR
NICKERSON, B
JORGENSON, JW
[J]. SCIENCE, 1989, 246 (4926) : 57 - 63

← 1 2 3 4 5 →