A SIMPLE METHOD FOR THE PURIFICATION OF HUMAN PERIPHERAL-BLOOD MONOCYTES - A SUBSTITUTE FOR SEPRACELL-MN

被引：70

作者：

DENHOLM, EM ^{[1
]}

WOLBER, FM ^{[1
]}

机构：

[1] UNIV MICHIGAN,MED CTR,DEPT PATHOL,ANN ARBOR,MI 48109

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 144卷 / 02期

关键词：

MONOCYTE; PURIFICATION; SEPRACELL;

D O I：

10.1016/0022-1759(91)90092-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient which matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 x 10(6) monocytes with a purity of 83% were obtained from 30-40 ml of blood. These monocytes were at least 97% viable and responded to chemotactic stimuli in comparable numbers to those prepared using Sepracell-MN

引用

页码：247 / 251

页数：5

共 14 条

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