AMINOPEPTIDASE-A IN HUMAN PLACENTA AND PREGNANT SERUM

被引:6
作者
OKUYAMA, T
ISHIURA, S
NOJIMA, M
TSUKAHARA, T
YANAGIDA, M
SUGITA, H
机构
[1] TOKYO METROPOLITAN TSUKIJI MATERN HOSP,DEPT OBSTET & GYNECOL,TOKYO,JAPAN
[2] JUNTENDO UNIV,SCH MED,DEPT OBSTET & GYNECOL,TOKYO 113,JAPAN
关键词
SERUM; AMINOPEPTIDASE-A;
D O I
10.1016/0009-8981(91)90074-M
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
The activities of serum aminopeptidases (APs) derived from the placenta rise during pregnancy. To confirm the localization of AP-A, which liberates N-terminal acidic amino acids, in placental tissue and to identify it in the serum of pregnant women, chromatographic separation was performed. When a placental extract was subjected to DEAE cellulose column chromatography, the Glu-MCA degradative activity, or AP-A, was separated into two entities. Inhibition testing revealed that the enzyme eluted in Fraction No. 20 from the DEAE-cellulose column consisted of typical AP-A with a molecular weight of approximately 500 000. When the Glu-MCA degradative activity was observed by filtering pregnant serum through HPLC TSK G3000 gel, the activity was found to be due to serum CAP rather than AP-A. What had been thought to be a biochemicophysiological action inherent in blood AP-A raised additional questions.
引用
收藏
页码:207 / 215
页数:9
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