INITIATION ON CHROMATIN TEMPLATES IN A YEAST RNA POLYMERASE-II TRANSCRIPTION SYSTEM

被引：50

作者：

LORCH, Y

LAPOINTE, JW

KORNBERG, RD

机构：

[1] Department of Cell Biology, Stanford Univ School of Medicine, Stanford

来源：

GENES & DEVELOPMENT | 1992年 / 6卷 / 12A期

关键词：

POLYMERASE-II TRANSCRIPTION SYSTEM; CHROMATIN TEMPLATES; YEAST; CYC1; PROMOTER;

D O I：

10.1101/gad.6.12a.2282

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Templates were prepared with either the TATA box or transcription start sites of the yeast CYC1 promoter in a nucleosome. In both cases, initiation in an unfractionated yeast RNA polymerase II transcription system was abolished by the nucleosome. The inhibition appeared to be relieved by the activator protein Gal4-VP16 binding to a site upstream of the promoter. Inhibition was not relieved, however, in a transcription system reconstituted from purified components, indicating a requirement for additional factors for the effect of Gal4-VP16.

引用

页码：2282 / 2287

页数：6

共 36 条

[1] THE TRANSCRIPTIONALLY-ACTIVE MMTV PROMOTER IS DEPLETED OF HISTONE H1
BRESNICK, EH
BUSTIN, M
MARSAUD, V
RICHARDFOY, H
HAGER, GL
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (02) : 273 - 278
[2] ACTIVATION OF YEAST POLYMERASE-II TRANSCRIPTION BY HERPESVIRUS VP16 AND GAL4 DERIVATIVES INVITRO
CHASMAN, DI
LEATHERWOOD, J
CAREY, M
PTASHNE, M
KORNBERG, RD
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (11) : 4746 - 4749
[3] CONAWAY JW, 1991, J BIOL CHEM, V266, P17721
[4] SEQUENCE-SPECIFIC ANTIREPRESSION OF HISTONE HL-MEDIATED INHIBITION OF BASAL RNA POLYMERASE-II TRANSCRIPTION
CROSTON, GE
KERRIGAN, LA
LIRA, LM
MARSHAK, DR
KADONAGA, JT
[J]. SCIENCE, 1991, 251 (4994) : 643 - 649
[5] YEAST HISTONE H4 N-TERMINAL SEQUENCE IS REQUIRED FOR PROMOTER ACTIVATION INVIVO
DURRIN, LK
MANN, RK
KAYNE, PS
GRUNSTEIN, M
[J]. CELL, 1991, 65 (06) : 1023 - 1031
[6] PURIFICATION AND LIPID-LAYER CRYSTALLIZATION OF YEAST RNA POLYMERASE-II
EDWARDS, AM
DARST, SA
FEAVER, WJ
THOMPSON, NE
BURGESS, RR
KORNBERG, RD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (06) : 2122 - 2126
[7] ELGIN SCR, 1988, J BIOL CHEM, V263, P19259
[8] FEAVER WJ, 1991, J BIOL CHEM, V266, P19000
[9] STATISTICAL POSITIONING OF NUCLEOSOMES BY SPECIFIC PROTEIN-BINDING TO AN UPSTREAM ACTIVATING SEQUENCE IN YEAST
FEDOR, MJ
LUE, NF
KORNBERG, RD
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1988, 204 (01) : 109 - 127
[10] FELSENFELD G, 1992, DNATURE, V355, P219

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