FATE OF A BACTERIUM, PSEUDOMONAS-PAUCIMOBILIS SS86, IN UPLAND FIELD

被引:8
作者
SENOO, K
WADA, H
机构
[1] The University of Tokyo, Bunkyo-ku, Tokyo
关键词
ENVIRONMENTAL RELEASE OF GEMS; FATE OF PSEUDOMONAS-PAUCIMOBILIS SS86; GAMMA-HCH; MPN METHOD; UPLAND FIELD;
D O I
10.1080/00380768.1990.10416796
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The fate of Pseudomonas paucimobilis SS86, which was isolated from an upland field where gamma-HCH (gamma-hexachlorocyclohexane, gamma-BHC, lindane) had been repeatedly applied for more than 10 years and which had a peculiar ability to decompose-gamma-HCH rapidly and completely, was studied using an upland field soil where gamma-HCH had never been applied previously. A localized area of the surface soil (10 cm thick and 30 cm in diameter) in the field plot was inoculated with P. paucimobilis SS86 along with gamma-HCH and the fate of the bacterium was monitored in several areas in the plot during the following 21 weeks. In the inoculated area, the number of bacteria increased, reached a maximum, and decreased afterwards. The bacterium seemed to prefer vertical movement to horizontal movement in the soil. It was confirmed that the genes responsible for gamma-HCH decomposition were not lost throughout the experimental period, though some of the genes were encoded in some plasmids and were not very stable when the bacterium multiplied rapidly in the absence of gamma-HCH.
引用
收藏
页码:593 / 598
页数:6
相关论文
共 20 条
[1]  
Acea M.J., Moore C.R., Alexander M., Survival and growth of bacteria introduced into soil, Soil Biol Biochem, 20, pp. 509-515, (1988)
[2]  
Acea M.J., Alexander M., Growth and survival of bacteria introduced into carbon-amended soil, Soil Biol Biochem, 20, pp. 703-709, (1988)
[3]  
Bentjen S.A., Fredrickson J.K., Van Volis P., Li S.W., Intact soil-core microcosms for evaluating the fate and ecological impact of the release of genetically engineered microorganisms, Appl. Environ. Microbiol, 55, pp. 198-202, (1989)
[4]  
Hamdi Y.A., Soil-water tension and the movement of, Rhizobia. Soil Biol. Biochem, 3, pp. 121-126, (1971)
[5]  
Hattori T., Microbial distribution and its variation in soil aggregates, Jpn J. Soil Sci. Plant Nutr, 37, pp. 302-304, (1965)
[6]  
Hattori T., Microbial habitat in soil, Jpn. Agric. Res. Q, 11, (1977)
[7]  
Jain R.K., Burlage R.S., Sayler G.S., Methods for detecting recombinant DNA in the environment, CRC Crit. Rev. Biotech, 8, pp. 33-84, (1988)
[8]  
Kiyohara H., Nagao K., Kouno K., Yano K., Phenanthrene-degrading phenotype of Alcaligenes faecalis AFK2, Appl. Environ. Microbiol, 43, pp. 458-461, (1982)
[9]  
Kiyohara H., Nagao K., Yano K., Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates, Appl. Environ. Microbiol, 43, pp. 454-457, (1982)
[10]  
Levin M.A., Seidler I.R., Borquin A.W., Fowle J.R., Barkay T., EPA developing methodsto assess environmental reiease, Bio/Technology, 5, pp. 38-45, (1987)