INOSINE-CONTAINING PRIMERS IN HUMAN PAPILLOMAVIRUS DETECTION BY POLYMERASE CHAIN-REACTION

被引：6

作者：

NOVELLI, G

GENNARELLI, M

DESANTIS, L

ANGELONI, P

DALLAPICCOLA, B

机构：

[1] UNIV ROME 2, DEPT PUBL HLTH & CELL BIOL, ROME, ITALY

[2] UNIV URBINO, I-61029 URBINO, ITALY

[3] HOSP IRCCS SAN GIOVANNI, CSS, ROTONDO, ITALY

[4] LAB CENT CRI, ROME, ITALY

来源：

BIOMEDICINE & PHARMACOTHERAPY | 1992年 / 46卷 / 04期

关键词：

POLYMERASE CHAIN REACTION; PAPILLOMAVIRUS; INOSINE;

D O I：

10.1016/0753-3322(92)90025-3

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A PCR protocol has been developed using inosine-containing primers for human papilloma virus (HPV) detection. The HPV16 L1 region from CaSki-positive cells was efficiently amplified by a single reaction and directly analyzed by agarose gel electrophoresis. The method was found to be sensitive and reproducible, and is easy to use for HPV typing and screening.

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页码：167 / 169

页数：3

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