THE USE OF PYROLYSIS MASS-SPECTROMETRY TO DETECT THE FIMBRIAL ADHESIVE ANTIGEN F41 FROM ESCHERICHIA-COLI HB101 (PSLM204)

被引：21

作者：

GOODACRE, R ^{[1
]}

BERKELEY, RCW ^{[1
]}

BERINGER, JE ^{[1
]}

机构：

[1] UNIV BRISTOL, SCH BIOL SCI, DEPT BOT, BRISTOL BS8 1UG, AVON, ENGLAND

来源：

JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS | 1991年 / 22卷 / 1-2期

关键词：

ANTIGEN; ESCHERICHIA-COLI HB101; ENTEROTOXIN; FIMBRIAE; PYROLYSIS;

D O I：

10.1016/0165-2370(91)85002-O

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Escherichia coli HB101 is a plasmidless, non-fimbriated bacterium. The plasmid pSLM204, which encodes for the production of the adhesive F41 antigen found in enterotoxigenic E. coli, was sub-cloned into E. coli HB101. It is possible to remove the F41 antigen from E. coli HB101(pSLM204) by physical shearing and to monitor its removal by electron microscopy and mannose-resistant haemagglutination with guinea pig erythrocytes. Pyrolysis-mass spectrometry can differentiate between E. coli HB101, HB101(pSLM204) and the latter strain after the removal of F41 fimbriae. This study shows that the difference observed is due largely to F41 antigens on the surface of strain HB101(pSLM204).

引用

页码：19 / 28

页数：10

共 21 条

[1] EVALUATION OF A LOW-COST, AUTOMATED PYROLYSIS MASS-SPECTROMETER [J].