TRANSFORMATION WITH KATG RESTORES ISONIAZID-SENSITIVITY IN MYCOBACTERIUM-TUBERCULOSIS ISOLATES RESISTANT TO A RANGE OF DRUG CONCENTRATIONS

被引：155

作者：

ZHANG, Y ^{[1
]}

GARBE, T ^{[1
]}

YOUNG, D ^{[1
]}

机构：

[1] HAMMERSMITH HOSP,MRC,TUBERCULOSIS & RELATED INFECT UNIT,DUCANE RD,LONDON W12 0HS,ENGLAND

来源：

MOLECULAR MICROBIOLOGY | 1993年 / 8卷 / 03期

关键词：

D O I：

10.1111/j.1365-2958.1993.tb01596.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isoniazid-resistant isolates of Mycobacterium tuberculosis were transformed with a plasmid vector carrying the functional catalase-peroxidase (katG) gene. Expression of katG restored full drug susceptibility in isolates initially resistant to concentrations ranging from 3.2 to >50 mug ml-1. Transformation with the corresponding katG gene from Escherichia coli resulted in low-level expression of catalase and peroxidase activities and conferred partial isoniazid sensitivity.

引用

页码：521 / 524

页数：4