DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN-B - A COMPARATIVE-STUDY OF ELISA AND ELIFA SYSTEMS

被引：6

作者：

DUPONT, H

THERASSE, J

PINON, JM

BINDER, P

机构：

[1] CTR ETUDES BOUCHET,BP 3,F-91710 VERT LE PETIT,FRANCE

[2] HOP MAISON BLANCHE,F-51100 REIMS,FRANCE

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 128卷 / 02期

关键词：

ELIFA; ELISA; Staphylococcal enterotoxin B;

D O I：

10.1016/0022-1759(90)90222-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunofiltration assay (ELIFA) sandwich methods have been compared for their ability to detect staphylococcal enterotoxin B (SEB) in biological samples. Using the avidin-biotin amplification system in the ELISA, high sensitivity was observed and staphylococcal protein A interference was completely prevented. In contrast, ELIFA was less sensitive than ELISA, but very easy to perform, fast and simple to read routinely. © 1990.

引用

页码：287 / 291

页数：5

共 7 条

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