EVIDENCE FOR PRESENCE OF PEPTIDE ALPHA-AMIDATING ACTIVITY IN PANCREATIC-ISLETS FROM NEWBORN RATS

被引：7

作者：

ZHOU, A ^{[1
]}

THORN, NA ^{[1
]}

机构：

[1] UNIV COPENHAGEN,BIOTECHNOL CTR NEUROPEPTIDE RES,DK-2200 COPENHAGEN N,DENMARK

来源：

BIOCHEMICAL JOURNAL | 1990年 / 267卷 / 01期

关键词：

D O I：

10.1042/bj2670253

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The peptide α-amidating activity of a homogenate of pancreatic islets from 5-7-day-old rats was investigated, using as substrate a glycine-extended tripeptide (D-Tyr-Val-Gly). The islet homogenates had a marked amidating activity, with a K(m) of 57 μM, a V(max.) of 185 pmol/h per mg and a pH optimum of 7.0. This activity was dependent on the presence of ascorbic acid (in the reduced form) and Cu2+, the optimum concentrations being 4 mM and 40 μM respectively. On fractionation of the homogenate, the highest specific activity was found in the soluble fraction. Exocrine pancreatic tissue showed very low levels of amidating activity.

引用

页码：253 / 256

页数：4

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