SIMPLIFIED APPROACH TO ENZYMATIC IDENTIFICATION OF OSTEOCLASTIC BONE-RESORPTION

被引：8

作者：

PARKINSON, IH

FAZZALARI, NL

DURBRIDGE, TC

MOORE, RJ

机构：

[1] The Division of Tissue Pathology, Institute of Medical and Veterinary Science, Adelaide, SA, 5000

来源：

JOURNAL OF HISTOTECHNOLOGY | 1991年 / 14卷 / 02期

关键词：

ACID PHOSPHATASE; BONE RESORPTION; HISTOCHEMISTRY; HISTOQUANTITATION; OSTEOCLASTS; UNDECALCIFIED BONE;

D O I：

10.1179/his.1991.14.2.81

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Osteoclastic bone resorption was estimated by a modified enzyme histochemical method for the localization of acid phosphatase in osteoclasts correlated with morphology. The results were compared with a standard biochemical assay of urinary hydroxyproline/creatinine ratio (OHP/Cr) to validate the method. The extent of active trabecular bone resorption estimated by the enzymatic method was greater than the extent of trabecular bone covered by classical multinucleate osteoclasts. We suggest that the enzyme method is a better histological indicator of the state of skeletal bone resorption than the morphological method because it is easier to perform and corresponds approximately in extent to the sum of inactive and active resorption as measured by morphological means.

引用

页码：81 / 83

页数：3

共 13 条

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