EPITOPE MAP OF 2 POLYCLONAL ANTIBODIES THAT RECOGNIZE AMYLOID LESIONS IN PATIENTS WITH ALZHEIMERS-DISEASE

被引：17

作者：

GHISO, J ^{[1
]}

WISNIEWSKI, T ^{[1
]}

VIDAL, R ^{[1
]}

ROSTAGNO, A ^{[1
]}

FRANGIONE, B ^{[1
]}

机构：

[1] NYU MED CTR,DEPT NEUROL,NEW YORK,NY 10016

来源：

BIOCHEMICAL JOURNAL | 1992年 / 282卷

关键词：

D O I：

10.1042/bj2820517

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two synthetic peptides with sequences identical with those of fragments of the extracellular domain of the Alzheimer's-disease amyloid precursor protein (APP) were used to raise antibodies. SP28 comprises positions 597-624 of the APP695 isoform, whereas SP41 extends towards the N-terminus (amino acids 584-624) and contains the entire SP28 peptide. Using e.l.i.s.a. and inhibition experiments we identified the two beta-turn-containing segments 602-607 and 617-624 as the epitopes recognized by anti-SP41 and anti-SP28 respectively. Both antibodies immunolabelled amyloid lesions in brains from Alzheimer's-disease patients and patients with related disorders, whereas they were unreactive in control brains. However, when probed on immunoblots, anti-SP28 failed to detect full-length APP from baculovirus-infected Sf9 cells, and anti-SP41 reacted weakly compared with other anti-APP antisera. The data suggest that these antibodies are directed to conformational epitopes not existent in the native molecules but present after alternative APP processing.

引用

页码：517 / 522

页数：6

共 42 条

[1] EXPRESSION OF ACTIVE SECRETED FORMS OF HUMAN AMYLOID BETA-PROTEIN PRECURSOR BY RECOMBINANT BACULOVIRUS-INFECTED INSECT CELLS [J].