LEUCINE-58 IN THE PUTATIVE 5TH HELICAL REGION OF HUMAN INTERLEUKIN (IL)-6 IS IMPORTANT FOR ACTIVATION OF THE IL-6 SIGNAL TRANSDUCER, GP130

被引：9

作者：

DEHEN, FD

BOS, HK

EBELING, SB

GROTZINGER, J

KURAPKAT, G

ROSEJOHN, S

AARDEN, LA

BRAKENHOFF, JPJ

机构：

[1] NETHERLANDS RED CROSS,BLOOD TRANSFUS SERV,CENT LAB,DEPT AUTOIMMUNE DIS,1066 CX AMSTERDAM,NETHERLANDS

[2] UNIV AMSTERDAM,CLIN & EXPTL IMMUNOL LAB,1066 CX AMSTERDAM,NETHERLANDS

[3] RHEIN WESTFAL TH AACHEN,DEPT BIOCHEM,W-5100 AACHEN,GERMANY

[4] UNIV MAINZ,PATHOPHYSIOL SECT,MED CLIN 1,W-6500 MAINZ,GERMANY

来源：

FEBS LETTERS | 1995年 / 369卷 / 2-3期

关键词：

INTERLEUKIN-6; STRUCTURE-FUNCTION ANALYSIS; GP130;

D O I：

10.1016/0014-5793(95)00741-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A model of the tertiary structure of human IL-6, derived from the crystal-structure of granulocyte-colony stimulating factor, reveals a 5th helical region in the loop between the first and second alpha-helix. To investigate the importance of this region for biological activity of IL-6, residues Glu-52, Ser-53, Ser-54, Lys-55, Glu-56, Leu-58 and Glu-60 were individually replaced by alanine. IL-6 . Leu-58Ala displayed a 5-fold reduced biological activity on the IL-6-responsive human cell lines XG-1 and A375. This reduction in bioactivity was shown to be due to a decreased capacity of the mutant protein to trigger IL-6 receptor-alpha-chain-dependent binding to the IL-6 signal transducer, gp130.

引用

页码：187 / 191

页数：5

共 30 条

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