A NOVEL MUTATION OCCURRING IN THE PHO80 GENE SUPPRESSES THE PHO4C MUTATIONS OF SACCHAROMYCES-CEREVISIAE

被引：15

作者：

OKADA, H ^{[1
]}

TOHE, A ^{[1
]}

机构：

[1] HIROSHIMA UNIV,DEPT FERMENTAT TECHNOL,HIROSHIMA 724,JAPAN

来源：

CURRENT GENETICS | 1992年 / 21卷 / 02期

关键词：

PHO; SACCHAROMYCES; PROTEIN-PROTEIN INTERACTION; REGULATION;

D O I：

10.1007/BF00318466

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have isolated suppressors of a PHO4c (a positive regulator) mutant which normally confers weak constitutivity for acid phosphatase production on the Saccharomyces cell. One dominant suppressor (PHO80-2) was found to be an allele of PHO80 (a negative regulator) that changes G to A, resulting in substitution of isoleucine for methionine 42 of the Pho80 protein. Substitution of valine (PHO80-3) or leucine (PHO80-4) for the same methionine by site-directed mutagenesis also suppressed PHO(c). Suppression by PHO80-2) did show some allele specificity. From these results we were able to delimit the region of PHO80 which may interact with the Pho4 protein.

引用

页码：95 / 99

页数：5

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