METASTATIC CONVERSION OF CELLS BY EXPRESSION OF HUMAN PAPILLOMAVIRUS TYPE-16 E6 AND E7 GENES

被引：32

作者：

CHEN, LP ^{[1
]}

ASHE, S ^{[1
]}

SINGHAL, MC ^{[1
]}

GALLOWAY, DA ^{[1
]}

HELLSTROM, I ^{[1
]}

HELLSTROM, KE ^{[1
]}

机构：

[1] FRED HUTCHINSON CANC RES CTR, SEATTLE, WA 98104 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 14期

关键词：

MALIGNANCY; TRANSFORMATION; TUMORIGENICITY; CANCER METASTASIS;

D O I：

10.1073/pnas.90.14.6523

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The human papillomavirus type 16 (HPV-16) is a DNA tumor virus highly-associated with cervical carcinoma. Viral DNA from HPV-16 is found in primary tumors and their metastatic lesions. To investigate the role of HPV-16 oncoproteins in the development of cancer metastasis, the E6 and E7 genes from HPV-16 were inserted into retrovirus and introduced into nonmetastatic mouse cell lines. Expression of either of the viral genes from HPV-16 made the cells metastatic in nude mice. In contrast, expression of the E6 and E7 genes of HPV type 6 (HPV-6b), which is frequently found in nonmalignant HPV-associated diseases, did not. The metastatic ability of cells transduced with viral genes of HPV-16 did not correlate with their growth rate or sensitivity to destruction by natural killer cells. Our results demonstrate that expression of oncogenic proteins of HPV-16 can cause tumor metastasis and implicate HPV-16 in an important role regarding the progression of HPV-associated human cancers.

引用

页码：6523 / 6527

页数：5

共 47 条

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