IDENTIFICATION OF ANTICOAGULANT ACTIVITIES IN THE SALIVARY-GLANDS OF THE SOFT TICK, ORNITHODOROS-SAVIGNYI

被引：41

作者：

GASPAR, ARMD

CRAUSE, JC

NEITZ, AWH

机构：

[1] Department of Biochemistry, University of Pretoria, Pretoria

来源：

EXPERIMENTAL & APPLIED ACAROLOGY | 1995年 / 19卷 / 02期

关键词：

HEMATOPHAGOUS; TICK; ORNITHODOROS SAVIGNYI; ANTICOAGULANT; FACTOR XA; THROMBIN;

D O I：

10.1007/BF00052551

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Salivary gland extracts of the sand tampan, Ornithodoros savignyi, prolonged the activated partial thromboplastin time (APTT) and prothrombin time (PT) significantly in a concentration-dependent manner. There was also a pronounced inhibition of human activated factor Xa (fXa) by salivary gland extracts. The salivary gland extracts inhibited chromogenic assays specific for both fXa and thrombin. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of the salivary gland proteins followed by elution of specific areas or bands from a polyvinylidene difluoride (PVDF)-membrane, showed that various anticoagulant factors are present when screened by means of the APTT assay. The most active component was associated with a band of M(r) of 14 kDa. Partial purification of this component was achieved using isoelectric focusing (IEF) and size-exclusion high-performance liquid chromatography (HPLC).

引用

页码：117 / 127

页数：11

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