PRECIPITATION OF NUCLEIC-ACIDS WITH POLY(ETHYLENEIMINE)

被引：44

作者：

CORDES, RM ^{[1
]}

SIMS, WB ^{[1
]}

GLATZ, CE ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,DEPT CHEM ENGN,231 SWEENEY HALL,AMES,IA 50011

来源：

BIOTECHNOLOGY PROGRESS | 1990年 / 6卷 / 04期

关键词：

D O I：

10.1021/bp00004a009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Removal of nucleic acids from cell extracts is a common early step in downstream processing for protein recovery. We report on the precipitation of nucleic acids from a homogenate of Saccharomyces cerevisiae by addition of the cationic polyelectrolyte poly (ethyleneimine) (PEI), focusing on the effect of PEI dosage on particle size, protein loss, and extent of nucleic acid removal in both batch and continuous mode. Better than 95 % removal of nucleic acids from yeast homogenates was achieved by means of precipitation with PEI with protein losses of approximately 15% with or without previous removal of cell debris. The coprecipitated protein is predominately large molecular weight material and exhibits both low and high isoelectric points. Such treatment does not aggregate the cell debris; size distribution of the precipitated particles from a continuous precipitator is very similar to that for protein precipitation. Copyright © 1990 American Institute of Chemical Engineers (AIChE)

引用

页码：283 / 285

页数：3

共 15 条

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