DETECTION OF SPECIFIC GLUCOSE-3-PHOSPHATASE ACTIVITY IN RAT-LIVER

被引：4

作者：

CANALES, J ^{[1
]}

BUITRAGO, F ^{[1
]}

FARALDO, A ^{[1
]}

CAMESELLE, JC ^{[1
]}

机构：

[1] UNIV EXTREMADURA,FAC MED,DEPT BIOQUIM & BIOL MOLEC & GENET,E-06080 BADAJOZ,SPAIN

来源：

FEBS LETTERS | 1994年 / 339卷 / 1-2期

关键词：

D-GLUCOSE-3-PHOSPHATE; GLUCOSE-3-PHOSPHATASE; SUGAR-3-PHOSPHATE; RAT LIVER; DIABETES;

D O I：

10.1016/0014-5793(94)80383-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sugar-3-phosphates are related to aspects of diabetes which depend on protein glycosylation events. Sorbitol-3-phosphate and fructose-3-phosphate occur in normal and diabetic individuals, and glucose-3-phosphate is a potential intermediate in their biosynthesis. Almost nothing is known about enzyme pathways for their metabolic turnover. We have found that part of the phosphohydrolytic activity on glucose-3-phosphate in rat liver supernatants corresponds to a specific, Mg2+-dependent, glucose-3-phosphatase much less or not active on other phosphate esters, including glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, fructose-6-phosphate and p-nitrophenyl-phosphate. This finding opens a route to a better understanding of the metabolism and role of sugar-3-phosphates.

引用

页码：55 / 58

页数：4

共 12 条

[1]

Bergmeyer H. U., 1974, METHOD ENZYMAT AN, V3, P1196

[2] C-13 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY OF MONOSACCHARIDES [J].