STUDIES ON THE GROWTH OF A THERMOTOLERANT YEAST-STRAIN, KLUYVEROMYCES-MARXIANUS IMB3, ON SUCROSE CONTAINING MEDIA

被引:26
作者
FLEMING, M
BARRON, N
MCHALE, L
MARCHANT, R
MCHALE, AP
机构
[1] Dept. of Biological and Biomedical Sciences, University of Ulster, Coleraine, Northern Ireland
关键词
D O I
10.1007/BF00130296
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A thermotolerant alcohol-producing yeast strain, Kluyveromyces marxianus IMB3 was shown to grow on sucrose (10% [w/v]) containing media at 45-degrees-C. Under such conditions the organism reached stationary phase within 20 hours and yielded ethanol concentrations in the region of 33g/L. During growth on sucrose containing media the organism was found to produce a cell- associated activity capable of hydrolysing sucrose. This activity was shown to have a Km of 5.0mM when sucrose was used as the substrate. In addition the enzyme was shown to have a pH optimum of 5.0 and a temperature optimum of 50-55-degrees-C and under those conditions the enzyme was shown to be relatively thermostable.
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页码:1195 / 1198
页数:4
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