PRODUCTION OF CHORISMATE MUTASE-PREPHENATE DEHYDROGENASE BY A STRAIN OF ESCHERICHIA-COLI CARRYING A MULTICOPY, TYRA PLASMID - ISOLATION AND PROPERTIES OF THE ENZYME

被引：13

作者：

BHOSALE, SB ^{[1
]}

ROOD, JI ^{[1
]}

SNEDDON, MK ^{[1
]}

MORRISON, JF ^{[1
]}

机构：

[1] AUSTRALIAN NATL UNIV, JOHN CURTIN SCH MED RES, DEPT BIOCHEM, CANBERRA, ACT 2601, AUSTRALIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1982年 / 717卷 / 01期

关键词：

D O I：

10.1016/0304-4165(82)90372-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A multicopy plasmid that contains the tyrosine operon was used to transform strains of E. coli K-12. The resultant strains yielded levels of chorismate mutase-prephenate dehydrogenase that were up to 5000-fold higher than that given by the parent strain and about 6-fold higher than that given by a tyrR strain. The production of enzyme fell when tetracycline was omitted from the growth medium because of the loss of the plasmid. The bifunctional enzyme was isolated in good yield by a simple purification procedure and shown to possess properties identical to those exhibited by the enzyme from a tyrR strains.

引用

页码：6 / 11

页数：6

共 20 条

[1] BHOSALE S, 1978, P AUST BIOCHEM SOC, V11, P24
[2] CAMAKARIS H, 1976, P AUST BIOCHEM SOC, V9, P4
[3] REGULATION OF AROMATIC AMINO-ACID BIOSYNTHESIS IN ESCHERICHIA-COLI K-12 - CONTROL OF THE AROF-TYRA OPERON IN THE ABSENCE OF REPRESSION CONTROL
CAMAKARIS, H
CAMAKARIS, J
PITTARD, J
[J]. JOURNAL OF BACTERIOLOGY, 1980, 143 (02) : 613 - 620
[4] PREPARATION AND PURIFICATION OF SODIUM PREPHENATE
DUDZINSKI, PK
MORRISON, JF
[J]. PREPARATIVE BIOCHEMISTRY, 1976, 6 (2-3): : 113 - 121
[5] CHORISMATE MUTASE-PREPHENATE DEHYDRATASE FROM ESCHERICHIA-COLI - ACTIVE-SITES OF A BIFUNCTIONAL ENZYME
DUGGLEBY, RG
SNEDDON, MK
MORRISON, JF
[J]. BIOCHEMISTRY, 1978, 17 (08) : 1548 - 1554
[6] GERHART JC, 1967, J BIOL CHEM, V242, P2886
[7] GIBSON F, 1968, BIOCHEM PREP, V12, P94
[8] GORNALL AG, 1949, J BIOL CHEM, V177, P751
[9] GUILFORD H, 1972, CHEM SCRIPTA, V2, P165
[10] PLASMID COLE1 AS A MOLECULAR VEHICLE FOR CLONING AND AMPLIFICATION OF DNA
HERSHFIE.V
BOYER, HW
YANOFSKY, C
LOVETT, MA
HELINSKI, DR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (09) : 3455 - 3459

← 1 2 →