CRESYL VIOLET - A RED FLUORESCENT NISSL STAIN

被引：44

作者：

ALVAREZBUYLLA, A

LING, CY

KIRN, JR

机构：

[1] The Rockefeller University, New York, NY 10021

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1990年 / 33卷 / 2-3期

关键词：

Counterstain; Cytoarchitecture; Fluorescence; Fluorescent tracer; Fluoro-Gold; Histology; Neurons;

D O I：

10.1016/0165-0270(90)90016-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cresyl violet is widely used by neurobiologists to visualize Nissl substance in bright-field microscopy. Here we describe a method for using this dye as a red fluorescent Nissl stain. Unlike the bright-field staining technique, fluorescent cresyl is compatible with other fluorescent dyes and tracers, such as fluorescein, Fluoro-Gold and Fast Blue. The procedure requires only minor modifications of routine bright-field cresyl staining, the most significant being dilution of the stain. Thus, fluorescent red cresyl violet is simple to implement and may be of general use in fluorescence microscopy. © 1990.

引用

页码：129 / 133

页数：5

共 20 条

[1] SIMPLE MICROCOMPUTER SYSTEM FOR MAPPING TISSUE-SECTIONS WITH THE LIGHT-MICROSCOPE
ALVAREZBUYLLA, A
VICARIO, DS
[J]. JOURNAL OF NEUROSCIENCE METHODS, 1988, 25 (02) : 165 - 173
[2] BIRTH OF PROJECTION NEURONS IN THE HIGHER VOCAL CENTER OF THE CANARY FOREBRAIN BEFORE, DURING, AND AFTER SONG LEARNING
ALVAREZBUYLLA, A
THEELEN, M
NOTTEBOHM, F
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (22) : 8722 - 8726
[3] ALVAREZBUYLLA A, 1988, J NEUROSCI, V8, P2707
[4] FLUORESCENT RETROGRADE NEURONAL LABELING IN RAT BY MEANS OF SUBSTANCES BINDING SPECIFICALLY TO ADENINE-THYMINE RICH DNA
BENTIVOGLIO, M
KUYPERS, HGJM
CATSMANBERREVOETS, CE
DANN, O
[J]. NEUROSCIENCE LETTERS, 1979, 12 (2-3) : 235 - 240
[5] 2 NEW FLUORESCENT RETROGRADE NEURONAL TRACERS WHICH ARE TRANSPORTED OVER LONG DISTANCES
BENTIVOGLIO, M
KUYPERS, HGJM
CATSMANBERREVOETS, CE
LOEWE, H
DANN, O
[J]. NEUROSCIENCE LETTERS, 1980, 18 (01) : 25 - 30
[6] INSITU HYBRIDIZATION USING PEG-EMBEDDED TISSUE AND RIBOPROBES - INCREASED CELLULAR DETAIL COUPLED WITH HIGH-SENSITIVITY
CLAYTON, DF
ALVAREZBUYLLA, A
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1989, 37 (03) : 389 - 393
[7] DIL AND DIO - VERSATILE FLUORESCENT DYES FOR NEURONAL LABELING AND PATHWAY TRACING
HONIG, MG
HUME, RI
[J]. TRENDS IN NEUROSCIENCES, 1989, 12 (09) : 333 - &
[8] GREEN FLUORESCENT LATEX MICROSPHERES - A NEW RETROGRADE TRACER
KATZ, LC
IAROVICI, DM
[J]. NEUROSCIENCE, 1990, 34 (02) : 511 - 520
[9] FLUORESCENT LATEX MICROSPHERES AS A RETROGRADE NEURONAL MARKER FOR INVIVO AND INVITRO STUDIES OF VISUAL-CORTEX
KATZ, LC
BURKHALTER, A
DREYER, WJ
[J]. NATURE, 1984, 310 (5977) : 498 - 500
[10] MORPHOLOGY AND LOCATION OF TECTAL PROJECTION NEURONS IN FROGS - A STUDY WITH HRP AND COBALT-FILLING
LAZAR, G
TOTH, P
CSANK, G
KICLITER, E
[J]. JOURNAL OF COMPARATIVE NEUROLOGY, 1983, 215 (01) : 108 - 120

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