CELL-SPECIFIC EXPRESSION OF THE MOUSE GLIAL FIBRILLARY ACIDIC PROTEIN GENE - IDENTIFICATION OF THE CIS-ACTING AND TRANS-ACTING PROMOTER ELEMENTS FOR ASTROCYTE-SPECIFIC EXPRESSION

被引:132
作者
MIURA, M
TAMURA, T
MIKOSHIBA, K
机构
[1] OSAKA UNIV,INST PROT RES,DIV REGULAT MACROMOLEC FUNCT,3-2 YAMADAOKA,SUITA,OSAKA 565,JAPAN
[2] NATL INST BASIC BIOL,DEPT BIOL REGULAT,DIV BEHAV & NEUROBIOL,OKAZAKI,AICHI 444,JAPAN
关键词
Astrocytes; Cell differentiation; Recombinant DNA; Transcription; Transfection;
D O I
10.1111/j.1471-4159.1990.tb03123.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Abstract: We have determined the whole promoter sequence and the transcriptional startpoint of the mouse glial fibrillary acidic protein (GFAP) gene and characterized the promoter function. We found that the cis elements for astrocyte specific expression are located within 256 bp from the transcription startpoint. We defined by DNase I footprinting assay three trans‐acting factor binding sites (GFI, GFII, and GFIII) using brain or C6 astrocytoma nuclear extracts. GFI, GFII, and GFIII have AP‐2, NFI, and cyclic AMP‐responsive element motifs, respectively. Mutations in GFII drastically decreased the promoter activity. Base substitution in GFI and GFIII abolished the cell‐specific expression, resulting in the GFAP promoter expression even in some non‐GFAP‐producing cells. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码:1180 / 1188
页数:9
相关论文
共 51 条
  • [1] PHORBOL ESTER INDUCIBLE GENES CONTAIN A COMMON CIS ELEMENT RECOGNIZED BY A TPA-MODULATED TRANS-ACTING FACTOR
    ANGEL, P
    IMAGAWA, M
    CHIU, R
    STEIN, B
    IMBRA, RJ
    RAHMSDORF, HJ
    JONAT, C
    HERRLICH, P
    KARIN, M
    [J]. CELL, 1987, 49 (06) : 729 - 739
  • [2] STRUCTURE OF THE MOUSE GLIAL FIBRILLARY ACIDIC PROTEIN GENE - IMPLICATIONS FOR THE EVOLUTION OF THE INTERMEDIATE FILAMENT MULTIGENE FAMILY
    BALCAREK, JM
    COWAN, NJ
    [J]. NUCLEIC ACIDS RESEARCH, 1985, 13 (15) : 5527 - 5543
  • [3] ACTIVITY OF 2 DIFFERENT SILENCER ELEMENTS OF THE CHICKEN LYSOZYME GENE CAN BE COMPENSATED BY ENHANCER ELEMENTS
    BANIAHMAD, A
    MULLER, M
    STEINER, C
    RENKAWITZ, R
    [J]. EMBO JOURNAL, 1987, 6 (08) : 2297 - 2303
  • [4] CHARACTERIZATION OF A SILENCER IN YEAST - A DNA-SEQUENCE WITH PROPERTIES OPPOSITE TO THOSE OF A TRANSCRIPTIONAL ENHANCER
    BRAND, AH
    BREEDEN, L
    ABRAHAM, J
    STERNGLANZ, R
    NASMYTH, K
    [J]. CELL, 1985, 41 (01) : 41 - 48
  • [5] BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025
  • [6] CALZONE FJ, 1987, METHOD ENZYMOL, V152, P611
  • [7] CAMPAGNONI AT, 1988, MOL NEUROBIOL, V2, P42
  • [8] NEGATIVE CONTROL OF LIVER-SPECIFIC GENE-EXPRESSION - CLONED HUMAN RETINOL-BINDING PROTEIN GENE IS REPRESSED IN HELA-CELLS
    COLANTUONI, V
    PIROZZI, A
    BLANCE, C
    CORTESE, R
    [J]. EMBO JOURNAL, 1987, 6 (03) : 631 - 636
  • [9] FIREFLY LUCIFERASE GENE - STRUCTURE AND EXPRESSION IN MAMMALIAN-CELLS
    DEWET, JR
    WOOD, KV
    DELUCA, M
    HELINSKI, DR
    SUBRAMANI, S
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (02) : 725 - 737
  • [10] ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI
    DIGNAM, JD
    LEBOVITZ, RM
    ROEDER, RG
    [J]. NUCLEIC ACIDS RESEARCH, 1983, 11 (05) : 1475 - 1489