PECULIAR PROPERTIES OF LIPASE FROM CANDIDA-PARAPSILOSIS (ASHFORD) LANGERON AND TALICE

被引：22

作者：

RIAUBLANC, A

RATOMAHENINA, R

GALZY, P

NICOLAS, M

机构：

[1] INRA,ENSA,CHAIRE MICROBIOL IND & GENET MICROORGANISMES,PL VIALA,F-34060 MONTPELLIER 1,FRANCE

[2] INST BIOL,INSERM,U65,F-34000 MONTPELLIER,FRANCE

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1993年 / 70卷 / 05期

关键词：

CANDIDA-PARAPSILOSIS; 1,3 DIGLYCERIDES; ESTERASE; ESTERS; LIPASE; LIPIDS; MONOGLYCERIDES; SPECIFICITY; SYNTHESIS;

D O I：

10.1007/BF02542583

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A new Candida parapsilosis lipase was isolated and studied. This enzyme was purified by hydrophobic chromatography on a phenyl-sepharose CL4B column followed by gel permeation on a Sephacryl S300 HR column. It was a 160 kg.mol-1 molecular-weight oligomeric enzyme. Optimal activity was obtained at 45-degrees-C and pH 6.5. The lipase activity toward various acylglycerols and esters was studied. The hydrolysis rate was greater for secondary acylesters than for primary acylesters. This lipase showed a high specificity for long-chain fatty acids and particularly for polyunsaturated fatty acids. This enzyme was able to catalyze the synthesis of various oleoylesters in aqueous medium.

引用

页码：497 / 500

页数：4

共 15 条

[11] MEASUREMENT OF PROTEIN USING BICINCHONINIC ACID [J].

SMITH, PK ;

KROHN, RI ;

HERMANSON, GT ;

MALLIA, AK ;

GARTNER, FH ;

PROVENZANO, MD ;

FUJIMOTO, EK ;

GOEKE, NM ;

OLSON, BJ ;

KLENK, DC .

ANALYTICAL BIOCHEMISTRY, 1985, 150 (01) :76-85

[12]

SOMOGYI M, 1952, J BIOL CHEM, V195, P19

[13] MICROBIAL LIPASES IN BIOTECHNOLOGY [J].

SZTAJER, H ;

ZBOINSKA, E .

ACTA BIOTECHNOLOGICA, 1988, 8 (02) :169-175

[14]

VANAUTRYVE P, 1991, OLEAGINEUX, V46, P29

[15] LIPASE MEDIATED SYNTHESIS OF LOW-MOLECULAR-WEIGHT FLAVOR ESTERS [J].

WELSH, FW ;

WILLIAMS, RE ;

DAWSON, KH .

JOURNAL OF FOOD SCIENCE, 1990, 55 (06) :1679-1682

← 1 2 →