IDENTIFICATION OF OCHRATOXIN-A IN FOOD SAMPLES BY CHEMICAL DERIVATIZATION AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：24

作者：

JIAO, YY ^{[1
]}

BLAAS, W ^{[1
]}

RUHL, C ^{[1
]}

WEBER, R ^{[1
]}

机构：

[1] MAX VON PETTENKOFER INST BUNDESGESUNDHEITSAMTES, POB 330013, W-1000 BERLIN 33, GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 595卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)85183-T

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The contamination of foods with ochratoxin A can be determined verv sensitively by high-performance liquid chromatography (HPLC) with fluorescence detection. A novel procedure is described to confirm OA-positive results quantitatively down to the HPLC detection limit of 0.1 ppb. For this, ochratoxin A in the sample extract is converted into its O-methylochratoxin A methyl ester derivative. which is identified subsequently by gas chromatography mass spectrometry negative-ion chemical ionization and multiple ion detection modes using the hexadeuterated O-methyl-d3-ochratoxin A methyl-d3 ester derivative as internal standard for quantification. In the analysis of more than 60 contaminated samples, the procedure was found to be very accurate.

引用

页码：364 / 367

页数：4

共 18 条

[1] MEASUREMENT OF OCHRATOXIN-A IN BARLEY EXTRACTS BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY [J].

ABRAMSON, D .

JOURNAL OF CHROMATOGRAPHY, 1987, 391 (01) :315-320

[2] OCHRATOXIN-A IN THE FOOD-CHAIN [J].

BAUER, J ;

GAREIS, M .

JOURNAL OF VETERINARY MEDICINE SERIES B-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1987, 34 (08) :613-627

[3]

COHEN H, 1986, J ASSOC OFF ANA CHEM, V69, P957

[4] DETERMINATION OF OCHRATOXIN-A IN HUMAN-MILK [J].

GAREIS, M ;

MARTLBAUER, E ;

BAUER, J ;

GEDEK, B .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1988, 186 (02) :114-117

[5]

HADLOK RM, 1989, MYKOTOXINE TIER STAM

[6]

HOWELL MV, 1981, J ASSOC OFF ANA CHEM, V64, P1356

[7]

JELINEK CF, 1989, J ASSOC OFF ANA CHEM, V72, P223

[8]

KROGH P, 1977, ACTA PATH MICRO IM B, V85, P238

[9]

Nowotny P., 1983, Chemie Mikrobiologie Technologie der Lebensmittel, V8, P29

[10]

PETKOVABOCHAROVA T, 1988, FOOD ADDIT CONTAM A, V5, P299, DOI 10.1080/02652038809373707

← 1 2 →