PULMONARY LYMPHOCYTE RECRUITMENT - DEPLETION OF CD8+ T-CELLS DOES NOT IMPAIR THE PULMONARY IMMUNE-RESPONSE TO INTRATRACHEAL ANTIGEN

被引:15
作者
CURTIS, JL
BYRD, PK
WARNOCK, ML
BECK, JM
KALTREIDER, HB
机构
[1] UNIV CALIF LOS ANGELES, VET ADM MED CTR,DEPT MED,MED SERV, PULM & CRIT CARE MED SECT 111D, SAN FRANCISCO, CA 94121 USA
[2] UNIV CALIF LOS ANGELES, CARDIOVASC RES INST, LOS ANGELES, CA 90024 USA
[3] UNIV MICHIGAN, VET ADM MED CTR, PULM SECT, ANN ARBOR, MI 48109 USA
[4] UNIV MICHIGAN, VET ADM MED CTR, DIV CRIT CARE MED, ANN ARBOR, MI 48109 USA
[5] UNIV MICHIGAN, DEPT INTERNAL MED, ANN ARBOR, MI 48109 USA
关键词
D O I
10.1165/ajrcmb/9.1.90
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
CD8+ T cells predominate in die lungs in hypersensitivity and human immunodeficiency virus-related lymphocytic pneumonitis, but their role in the immunopathogenesis of lung disease is unknown. We have shown that in immunized mice depleted of CD4+ T cells, CD8+ T cells are recruited into the lungs in response to intratracheal antigen challenge with sheep red blood cells (SRBC) (J. Clin. Invest. 1991; 88:1244-1254) or to pulmonary infection with Pneumocystis carinii (Am. J Respir. Cell Mol. Biol. 1991; 5:186-197), suggesting that recruitment of CD8+ T cells does not depend on CD4+ T cell-derived signals. Because CD8+ T cells themselves produce a variety of chemotactic and immunoregulatory cytokines, CD8+ T cells may be important participants in, and modulators of, pulmonary immune responses. To test this hypothesis, we examined the effects of CD8+ T cell depletion on the generation of a pulmonary immune response in vivo. We monitored the recruitment of mononuclear cells into lungs in the absence of CD8-dependent signals and measured the duration of pulmonary inflammation in the absence of suppressor CD8+ T cells. Primed mice were treated with anti-CD8 monoclonal antibody to deplete CD8+ T cells and subsequently were challenged intratracheally with 5 x 10(8) SRBC. At various times after challenge, total and differential cell counts and lymphocyte phenotypes were measured in bronchoalveolar lavage fluid by flow cytometry and lungs were scored histologically. We found that depletion of CD8+ T cells neither decreased recruitment of immune and inflammatory cells nor prolonged the pulmonary immune response. We conclude that: (1) CD8+ T cells do not produce signals necessary for cellular recruitment to the lungs in response to this particulate MHC class II-restricted antigen, and (2) termination of this pulmonary immune response does not require the action of CD8+ T cells. These results indicate that CD8+ T cells do not play a significant role in either the generation or the modulation of pulmonary immune responses to SRBC antigens.
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收藏
页码:90 / 98
页数:9
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