SELECTION OF TRANSFORMED PROTOPLAST-DERIVED ZEA-MAYS COLONIES WITH PHOSPHINOTHRICIN AND A NOVEL ASSAY USING THE PH INDICATOR CHLOROPHENOL RED

被引：9

作者：

KRAMER, C

DIMAIO, J

CARSWELL, GK

SHILLITO, RD

机构：

来源：

PLANTA | 1993年 / 190卷 / 04期

关键词：

CHLOROPHENOL RED; PHOSPHINOTHRICIN ACETYLTRANSFERASE; PROTOPLAST; TRANSFORMATION (SELECTION); ZEA (TRANSFORMED PROTOPLASTS);

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Phosphinothricin (PPT) inhibits glutamine synthetase in plant cells' resulting in an accumulation of ammonia that can be toxic. Bar, from Streptomyces hygroscopicus is a gene that codes for phosphinothricin acetyltransferase, an enzyme that detoxifies PPT by acetylation. We used polyethylene-glycol-mediated transformation to introduce bar into protoplasts of maize (Zea mays L.). With a novel assay utilizing the pH indicator chlorophenol red we identified transformants after only two to four weeks of selection on PPT.

引用

页码：454 / 458

页数：5

共 22 条

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