OPTIMIZATION OF CYTOTOXIC ASSAY BY TARGET-CELL RETENTION OF THE FLUORESCENT DYE CARBOXYFLUORESCEIN DIACETATE (CFDA) AND COMPARISON WITH CONVENTIONAL CR-51 RELEASE ASSAY

被引：65

作者：

PROVINCIALI, M

DISTEFANO, G

FABRIS, N

机构：

[1] Immunology Centre, Gerontology Research Department, Italian National Research Centres on Aging (I.N.R.C.A.), Ancona

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 155卷 / 01期

关键词：

CYTOTOXIC ASSAY; FLUOROMETRIC ANALYSIS; CARBOXYFLUORESCEIN DIACETATE;

D O I：

10.1016/0022-1759(92)90266-V

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes the comparison between a fluorimetric NK assay based on the target cell retention of fluorescent dye carboxyfluorescein diacetate (cFDA) and standard Cr-51 release assay. The results provide several suggestions to improve the cytotoxic assay based on the use of the fluorogenic substrate showing that the measurements of cFDA retained by target cells represent a method of evaluating cytotoxicity completely comparable to the Cr-51 release assay.

引用

页码：19 / 24

页数：6

共 9 条

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