CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF BACTERIAL ALGINATE LYASE

被引:10
作者
MIKAMI, B
HISANO, T
YAMASHITA, T
INOSE, T
TAKAGI, M
SAKAGUCHI, K
KIMURA, A
IMANAKA, T
MURATA, K
机构
[1] KYOTO UNIV,FOOD SCI RES INST,DEPT APPL MICROBIOL,UJI,KYOTO 611,JAPAN
[2] KYOTO UNIV,FOOD SCI RES INST,DEPT NEW FOOD DESIGN,UJI,KYOTO 611,JAPAN
[3] GUNZE CO LTD,KYOTO INST TECHNOL,DEPT BIOTECHNOL,AYABE 623,KYOTO,JAPAN
[4] OTSUKA PHARMACEUT CO LTD,DIV FOOD & BEVERAGE RES,TOKUSHIMA 77101,JAPAN
[5] OSAKA UNIV,FAC ENGN,DEPT APPL BIOTECHNOL,SUITA,OSAKA 565,JAPAN
来源
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1994年 / 77卷 / 06期
关键词
D O I
10.1016/0922-338X(94)90155-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Alginate lyase (EC 4.2.2.3) from a soil bacterium (Flavobacterium sp.), which was overexpressed in Bacillus subtilis, has been crystallized by the vapor-diffusion method. Tetragonal crystals were obtained using ammonium sulfate as a precipitant. The crystal was resistant to X-ray radiation damage and diffracted to about 2.2 Angstrom resolution. The diffraction pattern indicated that the crystal belongs to the orthorhombic system, space group P2(1)2(1)2(1) with unit-cell dimensions of a=82.7, b=142.1 and c=80.9 Angstrom. It is supposed that the asymmetric unit consists of two alginate lyase molecules.
引用
收藏
页码:691 / 692
页数:2
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